The Mouse Metabolic Phenotyping Center
at the Yale University School of Medicine

Analytical Core

	Dr. Gary Cline, Director, Analytical Core

Metabolic phenotyping of transgenic mice requires the ability to determine whole body, tissue specific, and cellular metabolic fluxes, and to delineate cellular mechanisms of signal transduction. The Analytical Core provides the expertise, technical resources, and instrumentation necessary to characterize perturbations in metabolism in transgenic mice strains, and serves as a resource lab for the analysis of samples generated during the course of experiments undertaken by the In Vivo, In Vitro, and NMR cores.

The Analytical Core is directed by Dr. Gary Cline.

The Analytical Core provides a facility for isotopic and chemical analysis of metabolites used to characterize physiologic parameters of metabolic flux rates, substrate turnover, intermediates that act as metabolic regulators, whole body composition, and energy expenditure. Steady-state and kinetic analysis of stable isotopic tracers provide a means to quantitatively evaluate metabolic fluxes and flux control. The facility is equipped with an array of state-of-the-art instrumentation, including GC-MS, LC-MS/MS, HPLC, and NMR, to provide the flexibility and power for analyses of metabolite concentrations and isotopic enrichments.